An Investigation into the Role of Antithrombin in Platelet Function

Time: 12:40 - 12:55

Date: 24 April 2018

Theatre: Dodder A


Platelets are anucleate cell fragments whose primary role is in haemostasis and the maintenance of vascular integrity. When activated, platelets release a multitude of proteins from their cytoplasm and preformed granules, known as the platelet proteome. Mass spectrometry studies have identified hundreds of proteins in this platelet releasate. The presence of the protein antithrombin in and its interaction with platelets is not well characterised. This project was designed to determine if antithrombin is part of the platelet proteome and to observe the effect which antithrombin has on platelets. This was achieved by using techniques such as a western blot, immunofluorescence, adhesion assays and flow cytometry.

Full identification of the proteins stored in and released by platelets when activated is important so that the role of these proteins can be determined. It is also crucial to develop a deeper knowledge of how plasma proteins interact with platelets so a better understanding of how platelets contribute to homeostasis can be made.


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